Transgenic Mouse Facility - a Mouse Genome Engineering Facility (MGEF)

The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.

The UC Irvine Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we also service academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients. The TMF's research associates have a combined 130 years of experience in generation of genetically engineered mice. Our experience can be your advantage.

Got a question? Email the TMF - we're here to help!

"CRISPR" Cas9/gRNA genome editing services

In certain cases CRISPR Cas9/gRNA mediated gene editing can accelerate projects to modify the mouse genome. As of January 2019, the TMF has successfully conducted 67 independent projects to modify the mouse genome via use of Cas9/gRNA, many of which involved use of IDT's ssODN or MegaMers, with 23 more currently in progress. See the Cas9/gRNA Mediated Genome Engineering Services page for details.

Of interest to TMF and Cancer Center users

Is your gRNA not mediating cutting ? Maybe inclusion of a 'TT' or 'GCC' sequence in the seed domain is the reason ? (2019) Cell Reports 26, p1098

Nucleosomes inhibit target cleavage by CRISPR-CAS9 in vivo - a possible reason why targeting efficiency varies from locus to locus. (2018) PNAS 38, p9351.

The Mice that Grow Human Tumours  - a brief news feature on utility of PDX mouse models (2018) Nature 560, p156

NCG - NOD CRISPR prkdc Il2r Gamma triple immunodeficient Mouse Model - A new mouse model for engratment of human normal and tumor tissues.

Genetically engineered mouse models in oncology research and cancer medicine. (2017) EMBO Molecular Medicine 9, p137.

Of mice and CRISPR : The post-CRISPR future of the mouse as a model system for the human condition. (2017) EMBO Reports 18, p187.

How can we assist you ?

• Finding and importing mice
• Need to import or export mice?  Use Mustrac - an on-line tool developed at UCI
• Making mutant mice
  • Cas9/gRNA-mediated genome engineering
  • Targeted mutations via homologous recombination in ES-cells
  • Inserting transgenes into the ROSA26 locus

• Designing a targeting vector
• Breeding
• Genotyping
• What strain of mice did this ES cell line come from? (PDF)