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The authors acknowledge the support of the Chao Family Comprehensive Cancer Center Transgenic Mouse Facility Shared Resource, supported by the National Cancer Institute of the National Institutes of Health under award number P30CA062203. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
The UC Irvine Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we also service academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients. The TMF's research associates have a combined 130 years of experience in generation of genetically engineered mice. Our experience can be your advantage.
Got a question? Email the TMF - we're here to help!
CRISPR/Cas9 genome-editing services
As of September 2020, the TMF has successfully conducted 94 independent projects to modify the mouse genome via use of CRISPR / Cas9 with 11 more in progress. See the CRISPR-Cas9 Mediated Genome Engineering Services page for details.
Of interest to TMF and Cancer Center users
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. (2020) Science Advances 6, doi: 10.1126/sciadv.abc9450
Near-Infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin (2019) eLife https://doi.org/10/7554/eLife.45801.001
p53 defies convention again: a p53 mutant that has lost tumor suppression but still can kill (2019) EMBO J. 38, e103322
Is your gRNA not mediating cutting ? Maybe inclusion of a 'TT' or 'GCC' sequence in the seed domain is the reason ? (2019) Cell Reports 26, p1098
Nucleosomes inhibit target cleavage by CRISPR-CAS9 in vivo - a possible reason why targeting efficiency varies from locus to locus. (2018) PNAS 38, p9351.
Transgenic Mouse Models in Cancer Research - a review of methods to generate mouse models of human cancer and examples of their use. (2018) Frontiers in Oncology 8:268
How can we assist you ?
- Finding and importing mice
- Need to import or export mice? Use Mustrac - an on-line tool developed at UCI
- Making mutant mice