Transgenic Mouse Facility - a Mouse Genome Engineering Facility (MGEF)

To ackowledge the TMF in your publications, copy and paste the language below

The authors acknowledge the support of the Chao Family Comprehensive Cancer Center Transgenic Mouse Facility Shared Resource, supported by the National Cancer Institute of the National Institutes of Health under award number P30CA062203. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

The UC Irvine Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we also service academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients. The TMF's research associates have a combined 130 years of experience in generation of genetically engineered mice. Our experience can be your advantage.

Got a question? Email the TMF - we're here to help!

CRISPR/Cas9 genome-editing services

As of April 2021, the TMF has successfully conducted 102 independent projects to modify the mouse genome via use of CRISPR / Cas9 with 14 more in progress. See the CRISPR-Cas9 Mediated Genome Engineering Services page for details.

Of interest to TMF and Cancer Center users

The use of CRISPR/Cas9-based gene editing strategies to explore cancer gene function in mice. (2021) Current Opinion in Genes & Development 66:57-62.

CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy. (2020) Science Advances 6, doi: 10.1126/sciadv.abc9450

Near-Infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin (2019) eLife https://doi.org/10/7554/eLife.45801.001

p53 defies convention again: a p53 mutant that has lost tumor suppression but still can kill (2019) EMBO J. 38, e103322

Is your gRNA not mediating cutting ? Maybe inclusion of a 'TT' or 'GCC' sequence in the seed domain is the reason ? (2019) Cell Reports 26, p1098

Nucleosomes inhibit target cleavage by CRISPR-CAS9 in vivo - a possible reason why targeting efficiency varies from locus to locus. (2018) PNAS 38, p9351.

Transgenic Mouse Models in Cancer Research - a review of methods to generate mouse models of human cancer and examples of their use. (2018) Frontiers in Oncology 8:268

The Mice that Grow Human Tumours  - a brief news feature on utility of PDX mouse models (2018) Nature 560, p156

NCG - NOD CRISPR prkdc Il2r Gamma triple immunodeficient Mouse Model - A new mouse model for engraftment of human normal and tumor tissues.

Genetically engineered mouse models in oncology research and cancer medicine. (2017) EMBO Molecular Medicine 9, p137.

Of mice and CRISPR : The post-CRISPR future of the mouse as a model system for the human condition. (2017) EMBO Reports 18, p187.

How can we assist you ?

• Finding and importing mice
• Need to import or export mice?  Use Mustrac - an on-line tool developed at UCI
• Making mutant mice
  • Cas9/gRNA-mediated genome engineering
  • Targeted mutations via homologous recombination in ES-cells
  • Inserting transgenes into the ROSA26  and H11 loci

• Designing a targeting vector
• Breeding
• Genotyping
• What strain of mice did this ES cell line come from? (PDF)